Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique widely used in the detection of target nucleic acid. The kit provides a simple solution for LAMP and RT-LAMP assays for DNA and RNA detection. LAMP and RT-LAMP are common isothermal amplification techniques, which use LAMP-specific primers (provided by user) and strand displacement polymerase to rapidly detect target nucleic acid. The kit contains 2.5×LAMP Mix and RT-LAMP enzyme Mix, in which the 2.5×LAMP Mix contains reaction buffer, fluorescent dye, Mg2+, dNTPs, etc., and is used together with fluorescence quantitative PCR instrument or thermostatic fluorescence detector. When used, only primers and templates need to be added for nucleic acid isothermal amplification.
· Efficient amplification reaction: Taq enzymes with excellent performance
and reverse transcriptase ensure high reaction efficiency.
· Simple and fast operation due to the bi-component product form, with
operational errors greatly reduced.
· Good compatibility: highly applicabile to RNA templates of different sample types.
Shipping and Storage Conditions
The kit needs to be transported frozen and the components of the kit should
be stored away from light under -18°C.