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The NEST Glass Bottom plates are designed for use with confocal, high-resolution, differential interference contrast, polarized light, and phase contrast microscopes for cell observation, supporting live cell imaging with a specially designed bottom for easy grip, round cover glass inserts for an attractive appearance, medical-grade adhesive to ensure non-cytotoxicity, E-beam sterilization to SAL 10⁻⁶, and construction from high-clarity 100% virgin polystyrene and glass with high-quality, standard-thickness cover glass.