Cell Culture Inserts, PC, 12 Nested Inserts + 24 Well Plate, Non-Treated, 8μm, Individually Packaged in Peelable Tyvek Film & Blister (Cat. No. 725311)
Key Features
24 Well Non-Treated - Culture Plates (702011)
Inserts have translucent, 8.0 μm pore, polycarbonate membrane
12 Inserts included with each plate
12 plates per pack, 48 plates total per case
Individually Wrapped - 1 per blister pack - 48 plates and 576 inserts per case
Insert Diameter - 6.5mm
Plate volume per well - 0.6mL
Inner volume of insert - 0.1mL
Growth area of insert membrane - 0.33 cm²
Nominal pore density 1x10⁵ pores per cm²
Product Details
PC membrane inserts are ideal for tumor cell migration and invasion studies, hematostaxis or endothelial cell migration assays and co-culture applications.
Create a cell culture environment that closely mimics in vivo conditions.
Inserts allow polarized cells to perform metabolic functions more naturally by allowing nutrient uptake from both the apical and basolateral sides
Grow cells in co-culture systems either with direct cell-to-cell interaction or separated without physical contact
Membrane requires staining to be visible
The membrane features a low adsorption rate, minimizing the loss of small molecular proteins and other compounds
PC is compatible with common solvents used for fixation and staining
8.0 μm ideal for culture of leukocytes, tumor derived cells and neurons
Quality Details
USP grade VI virgin polystyrene
USP grade VI virgin polycarbonate
Manufactured in cGMP (21 CFR part 820/821) compliant factory
Produced in ISO 13485:2016 and ISO 9001 facility
Molded and packed in ISO 7 cleanroom
E-beam sterilized and lot tested to SAL 10^-6
Lot tested free of pyrogens USP 85
Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
Lot tested free DNase/RNase and DNA
Lot tested free BSE/TSE
Certified free of animal products
Cell and tissue culture technologies are increasingly essential in both basic research and applied life sciences. To better replicate in vivo conditions, advanced culture vessels and specialized surface treatments for cell adhesion are continually being developed, particularly for the growth of sensitive or specialized cell lines.
Among these innovations, permeable supports with microporous membranes have become a key tool. By allowing molecular exchange at both the apical and basal surfaces, they promote the growth of polarized cells and support secretion and absorption in a way that closely mirrors natural metabolic processes. This creates a more physiologically relevant environment, making permeable supports an effective solution for cultivating complex cell types.
Cell attachment on permeable supports (membranes) is sensitive to the initial seeding density. For first-time use, test several seeding densities to determine optimal growth.
During cultivation, add or remove liquid carefully through the gap between the insert and the well wall. Pipette slowly to avoid damaging the membrane.
Pre-incubating the permeable support in culture medium before seeding cells improves attachment and distribution.
Guidelines for Use
Pre-equilibration: Add culture medium to the wells of the multi-well plate, insert the membrane support, and place the plate in the incubator for at least 1 hour or overnight before seeding cells.
Cell seeding: After pre-equilibration, add the cell-containing medium directly into the insert.
Medium maintenance: Regularly check the medium volume in both the insert and the well. Replenish with fresh medium as needed.
Fixation and staining: Cells can be fixed and stained directly in the insert using standard cytological methods. Do not use solvents that may dissolve the PC membrane.
Long-term storage: After fixation and staining, the membrane can be carefully cut from the insert with a scalpel for long-term preservation.
