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Cell Culture Inserts, PET, 12 Nested Inserts + 24 Well Plate, TC Treated, 0.4μm, Opaque, ndividually Packaged in Peelable Tyvek Film & Blister (Cat. No. 725121)
Key Features
Product Details
Quality Details
Cell and tissue culture technologies are increasingly essential in both basic research and applied life sciences. To better replicate in vivo conditions, advanced culture vessels and specialized surface treatments for cell adhesion are continually being developed, particularly for the growth of sensitive or specialized cell lines.
Among these innovations, permeable supports with microporous membranes have become a key tool. By allowing molecular exchange at both the apical and basal surfaces, they promote the growth of polarized cells and support secretion and absorption in a way that closely mirrors natural metabolic processes. This creates a more physiologically relevant environment, making permeable supports an effective solution for cultivating complex cell types.
| Technical Specifications |       |
|---|---|
| Number of Wells | 24 Well |
| Pore Size | 0.4um |
| Membrane Material | PET |
| Surface Treatment | Tissue Culture Treated |
| Sterility | Sterile, Individually Wrapped In Peelable Film |
| Subscription Enabled | Yes |
Attention
Cell attachment on permeable supports (membranes) is sensitive to the initial seeding density. For first-time use, test several seeding densities to determine optimal growth.
During cultivation, add or remove liquid carefully through the gap between the insert and the well wall. Pipette slowly to avoid damaging the membrane.
Pre-incubating the permeable support in culture medium before seeding cells improves attachment and distribution.
Guidelines for Use
Pre-equilibration: Add culture medium to the wells of the multi-well plate, insert the membrane support, and place the plate in the incubator for at least 1 hour or overnight before seeding cells.
Cell seeding: After pre-equilibration, add the cell-containing medium directly into the insert.
Medium maintenance: Regularly check the medium volume in both the insert and the well. Replenish with fresh medium as needed.
Fixation and staining: Cells can be fixed and stained directly in the insert using standard cytological methods. Do not use solvents that may dissolve the PC membrane.
Long-term storage: After fixation and staining, the membrane can be carefully cut from the insert with a scalpel for long-term preservation.
