Nest BioFactory 5 Chamber; 1 Wide Plug Seal Cap ; Total+ 1 Narrow Plug Seal Cap, culture area: 3175cm2; TC; Sterile (Cat.No. 773204)
Key Features
Five-layer
3175 cm² growth area
1 per bag, 4 units per case
TC treated - plasma etched for adherent cells
2 Wide Plug caps attached
Also includes, 4 plug and 4 vent caps per flask, 8 total of each per case
USP grade VI virgin polystyrene
Sterile
Equivalent to Corning CellSTACK® 3313
Product Details
Individually packaged in double-layer sterile bags
Flask layers are ultrasonically welded together without adhesives or additives. This creates a strong, gas-tight seal between layers without introducing leachables and extractables that could cause cell culture abnormalities
Two port mouth options available. Wide mouth (26mm) ports make it easy to pour media and reach all layers of the flask. Narrow mouth (12mm) ports are designed to fit aseptic transfer caps for sterile transfer of cells and media into and out of the flasks
Optional threaded caps are offered with chemically resistant, heat-sealable flexible TPE tubing, enabling aseptic transfer of media and cells
Dimensionally and geometrically identical in design to Corning CellSTACK® flasks
Compatible with Corning accessories, caps and equipment
100% of flasks are tested for leakage via pressure testing before shipping
Clear lot number for batch traceability
Suitable for large-scale cultivation of adherent cells
Large growth area—cultivate a high volume of cells in a single operation while minimizing the risk of contamination
Compatible with automated equipment. Especially important for larger 40-layer flasks
Sterility Guarantee — Utilizing an imported Rhodotron® TT200 electron accelerator from Belgian IBA and an in-house irradiation facility
Sterile to SAL 1 X 10^-6
Extensively tested and validated for leachables, extractables, particle counts, nucleases, nucelotides, endotoxins, pyrogens, TSE/BSE etc., making Nest Biofactories suitable for end point pharmaceutical manufacturing. Full validation dossiers are available on request
Quality Details
Manufactured in cGMP (21 CFR part 820/821) compliant factory
Produced in ISO 13485:2016 and ISO 9001 facility
Molded and packed in ISO 7 cleanroom
E-beam sterilized and lot tested to SAL 10^-6
Lot tested free of pyrogens USP 85
Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
Lot tested free DNase/RNase and DNA
Lot tested free BSE/TSE
Certified free of animal products
Full validation dossier available upon request
Nest Biofactory™ Systems are compact, multi-layer, single-use cell culture platforms designed for affordable and reliable cell culture scale-up. Ideal for medium- to large-scale research and industrial production, they support the production of cell-based products such as vaccines, monoclonal antibodies, proteins and other biopharmaceuticals. Nest Biofactories are based on the Corning CellSTACK® line of multi-layer tissue culture flasks. They are identical in geometry and dimensions for similar performance. However, Nest flasks are free of leachables and extractables that could cause cell culture irregularities. Layers are ultrasonically welded together without adhesives or additives, greatly improving lot to lot reproducibility.
