Fetal Bovine Serum

Fetal bovine serum (FBS) is the most widely used supplement in in vitro mammalian cell culture. It covers almost all the components

needed for research in the life sciences industry, providing almost all the necessary ingredients for adherent and suspension cell culture,

most of which have not yet been chemically defined, such as growth factors, attachment factors, transport proteins, lipids, and hormones.

FBS is commonly used for cell line proliferation and primary culture, and is widely used in b asic research, target/drug discovery, drug

development, and clinical research.

FBS is extracted from the coagulated whole blood collected by puncturing the heart of the fetus. The serum is immediately centrifuged after

collection, frozen, and transported to the processing plant. Upon arrival at the processing plant, the serum is thawed and passed through

three 100nm (0.1μm) sterilizing filters, and then bottled through a sterile bottling process.

All batches of NEST® FBS undergo strict sterility, mycoplasma, and virus testing. Each batch of serum will also undergo cell growth, plate

efficiency, and clone efficiency tests on the following cell lines: HeLa, L929, SP2/O-AG14, MRC-5. Certified serum also need to undergo a

series of additional tests, including endotoxins, chemical components, protein electrophoresis, and radioimmunodiffusion tests

Get Quotation

Cat.NO.

Introduction

Source:

An important factor to consider in serum quality is the source of the serum, therefore, the traceability of

the serum is crucial. Each production batch of NEST® FBS is strictly controlled, starting from serum

collection, through all processing and production stages, to final packaging. NEST® guarantees the

accuracy of the geographic source of the serum and all other data declared on the Certificate of Analysis(COA).

Filtering and Packaging:

The raw serum is filtered through three 0.1μm sterile filters. The filtered serum is thoroughly mixed to

ensure uniformity. NEST® products are packaged through a sterile filling process, each step of which is

executed to ensure that the product complies with industry sterility assurance level standards SAL=10-4

(i.e., during the manufacturing process, the bacterial and fungal contamination level of the product

does not exceed 1 in 10,000 units). The filtering and bottling are carried out in a positive-pressure

HEPA-filtered controlled environment room.

Storage and Transportation Conditions

The storage conditions, batch number, and expiration date of NEST® FBS are indicated on the label. The storage condition is -20°C, and

the shelf life is 60 months. When the product is stored correctly, the best product performance can be guaranteed. It needs to be

transported at -20℃ or on dry ice.

Related Reference Tools