Cell Culture Insert
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Cell Culture Insert
Cell and tissue culture technologies have an increasing importance in the fields of basic and applied life science. New culture vessels and new surfaces for cell adsorption are continuously emerging, in order to simulate the internal environment as much as possible for culture of some special cell lines. Logically, using permeable supports with a microporous membrane becomes the basic method for culturing these cells. Permeable supports may effectively improve the culture of polar cells, because these supports allow cells to secrete on and absorb molecules from their basal and apical surfaces to metabolize in a more natural way, as well as to stimulate the in vivo environment to the maximum extent for culturing of some special cell lines.
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Cat.NO.
Introduction
• Sterilized by E-beam, SAL=10-6.
• Vacuum Plasma tissue culture treatment.
• Non-Pyrogenic, DNase/Rnase free.
• A rich selection of matching plates: 6-well, 12-well, 24-well.
• Passed the USP VI toxicity test.
• Cell culture plates are made of high clarity, 100% virgin polystyrene.
• Clear lot number for batch traceability.
• Innovative edge design for convenient sample loading.
• Low protein binding to ensure accurate results.
• Compatible with most solvents used for fixing and staining.




Attention：
• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
•During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
•Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.


Guidelines for us：
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight. • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.