1.Prepare cell suspension of the required concentration, then mix it with medium evenly in a container. A volume of 30-50ml per layer is recommended .
2.Slowly add the mixed solution into the 5-layer Cell Culture Flask with a serological pipette. To avoid foam or bubbles, it is recommended to set the pipette firmly against the wall, enable the stream to flow along the slope, and save a little liquid in the pipette each time.
The most recent issue introduces the NEST BioFactory, a cleverly designed vessel that facilitates the growth of adherent cells for large-scale production in industries such as vaccines, monoclonal antibodies, and biopharmaceuticals. The BioFactoryTM maximizes culture area while minimizing factory footprint, resulting in a significant boost in cost-effective production capacity without altering the kinetic conditions of cell growth. The episode explores the role of BioFactory in various industries, its usage stages, and how to operate it for maximum value.
Dear customers,
Thank you very much for your trust on NEST products.
The size of NEST PET/PETG Erlenmeyer flask are optimized to improve the product performance.
Still in search of an ideal large storage container for liquid? Fret that the container at hand can’t be sterilized? NEST EXTRA LARGE Carboy is what you need, if you have a special requirement of large volume. It is available in specifications of 10L and 20L. And the raw material of polypropylene can satisfy your demand for autoclaving.
As for the cryo preservation research, NEST has been designing and refining its cryo presevation product portfolios for users. First, three kinds of Cryogenic Vials: Cryogenic Vials (No codes), 2D Matrix Cryogenic Vial (Barcode), Three-code-in-one SBS Cryogenic Vial(Numeric code, Barcode, QR code) were upgraded. Now, Two new products are coming along with the new version of Cryogenic Vials: Vertical Cryopreservation Rack and Defroster. A more complete product portfolio for more convenient usage and better user experience is at birth now!
Recently, the nasal spray COVID-19 vaccine, VectorFlu ONE, was approved for emergency use by the national authorities in the Chinese mainland. The vaccine has been jointly developed by Hong Kong University, Xiamen University and Wantai Biopharmaceutical Company. The nasal spray vaccine functions by NEST Pre-filled Disposable Intranasal Atomization Device.
About Cell Cryopreservation
Cell cryopreservation is a long-term cell storage technique that places cells in a low-temperature environment to slow down its metabolism. Placing and storing cells in liquid nitrogenof gas phase at -196°C can temporarily suspend cells’ growth, and preserve their certain cellular properties in preparation of later thawing when needed. For cryopreservation, NEST offers three kinds of Cryogenic Vials: 1. Regular Cryogenic Vials (No codes) 2. 2D Matrix Cryogenic Vial (Barcode) 3. Three-code-in-one SBS Cryogenic Vial. (Numeric code, Barcode, QR code)
