Fetal bovine serum (FBS) is the most widely used supplement in in vitro mammalian cell culture. It covers almost all the components needed for research in the life sciences industry, providing almost all the necessary ingredients for adherent and suspension cell culture, most of which have not yet been chemically defined, such as growth factors, attachment factors, transport proteins, lipids, and hormones. 

FBS is commonly used for cell line proliferation and primary culture, and is widely used in b asic research, target/drug discovery, drug development, and clinical research.

FBS is extracted from the coagulated whole blood collected by puncturing the heart of the fetus. The serum is immediately centrifuged after collection, frozen, and transported to the processing plant. Upon arrival at the processing plant, the serum is thawed and passed through three 100nm (0.1μm) sterilizing filters, and then bottled through a sterile bottling process.

All batches of NEST® FBS undergo strict sterility, mycoplasma, and virus testing. Each batch of serum will also undergo cell growth, plate efficiency, and clone efficiency tests on the following cell lines: HeLa, L929, SP2/O-AG14, MRC-5. Certified serum also need to undergo a series of additional tests, including endotoxins, chemical components, protein electrophoresis, and radioimmunodiffusion tests