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Molecular diagnostics
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Molecular diagnostics
核酸提取
2022-11-30
核酸提取是指将核酸(DNA和RNA)从细胞中提取出来的过程,总的来说包括细胞裂解、核酸纯化及后续核酸浓度、纯度测定的步骤。根据原始样品的种类和核酸产物后续的各种应用目的,细胞裂解和核酸纯化步骤有不同的处理方法和要求。
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核酸检测
2022-11-30
核酸浓度与纯度检测最简单常用的仪器是微量紫外分光光度计,代表为NanoDrop。NanoDrop分光光度计利用分子的紫外吸收特性来测定样品浓度。核酸吸收峰为260nm,蛋白样品吸收峰为280nm。此外,很多提取过程中残留的污染物通常在280nm和230nm处有吸收峰。[ NanoDrop Spectrophotometer Resources | Thermo Fisher Scientific - CN]样品浓度与吸光度成正比关系(Beer-Lambert Law),因此通过测定吸收峰就可以得出核酸样品的浓度。
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