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McAb(monoclonal antibody)
McAb(monoclonal antibody)
Introduce
Medium preparation and storage
Cell culture
Clarification
Harvesting
Buffer preparation
Virus inactivation/removal
Stock solution preparation
Formulation and packaging
Vaccine
Virus culture
Harvesting
Inactivation
Buffer preparation
Concentration/dialysis
Sterilization and filtration
Formulation and packaging
Cell expansion and washing
Cell culture
Medium preparation and storage
Cell therapy
Cell isolation
Cell activation
Cell modification
Quality control
Cryopreservation
ADC
Medium preparation and storage
Cell culture
Protein purification
Small molecule processes (conjugation)
Formulation and packaging
Molecular diagnostics
单克隆抗体
2022-11-23
抗体是脊椎动物为应对外来入侵蛋白(抗原),保护机体不被伤害而分泌的、在体内进行循环的蛋白质分子,是机体体液免疫的主要参与者。抗体又被称为免疫球蛋白(immunoglobulin,Ig),通过电泳技术对血清蛋白进行精确的分析,发现大部分的抗体蛋白都集中在伽马球蛋白(gamma globulin)所在的条带,因此抗体也被称为免疫球蛋白。[血清蛋白通过物理的溶解度定义,可以被简单的区分为白蛋白(albumin)和球蛋白(globulin)]
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培养基配置与储存
2022-11-23
根据配置体积,选择合适的灭菌后容器,在容器中倒入约90%体积的超纯水,将培养基干粉全部倒入该容器中,用少量超纯水将袋内残留粉末洗出。
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细胞侵袭和迁移
2022-11-02
实验准备: 细胞,24孔板,细胞小室(8μm,24孔板专用),ECM Gel,10%FBS+培养基,无血清培养基,10 μ、200 μL、1000 μL移液器及配套枪头,1.5 mL EP管,冰盒
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细胞冻存与复苏
2022-11-02
一、如何处理收到的细胞 1.新买的或惠赠的细胞如何处理? 不管买的细胞、惠赠的细胞,刚拿到手应赶紧做这几件事:检测瓶子是否破裂;培养基是否漏出,是否浑浊;是否有支原体污染;迅速扩增冻存。
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万能的间充质干细胞
2022-10-26
多年来对干细胞的定义不断进行修正,并从不同的层面上来进行定义?。目前大多数生物学家和医学家认为干细胞是来自于胚胎、胎儿或成人体内具有在一定条件下无限制自我更新与增殖分化能力的一类细胞,能够产生表现型与基因型和自己完全相同的子细胞,也能产生组成机体组织、器官的已特化的细胞,同时还能分化为祖细胞?。
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干细胞成骨诱导分化
2022-10-25
间充质干细胞属于成体干细胞,可在体外通过不同的方法干预下下诱导分化为骨、软骨及其他结缔组织,因其来源广泛,分离无创伤,较低的免疫原性、生长周期短等特点,是组织工程种子细胞的重要来源。干细胞的诱导分化一方面是干细胞鉴定的主要方法,另一方面也是干细胞功能学研究的主要途径。采用体外条件培养基诱导培养下,人间充质干细胞应能够分化为成脂肪、骨、软骨细胞。成骨分化经茜素红染色法鉴定。
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