Ultra-Low Attachment Surface
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Ultra-Low Attachment Surface
NEST ultra-low attachment surface efficiently allows cells to form suspended 3D spheres, addressing the problems of abnormal flattening, division, and loss of differentiation phenotype that occur when cultured in vitro on a flat culture surface.
The ultra-low attachment surface is coated with a special hydrogel that inhibits specific and non-specific solidification. It is biologically inert, non-cytotoxic, and non-degradable, making it an ideal material for various culture vessels.
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Introduction

Features

• Facilitates the formation of 3D multicellular spheres.

• Keeps cells suspended and non-adherent.

• Prevents specific stem cell differentiation induced by adhesion.

• Inhibits anchorage-dependent cells division.

• Reduces binding of attachment proteins and serum proteins to substrates.

Application

• Formation of 3D multicellular spheres, such as embryoid bodies and tumor spheroids.

• Formation of neurospheres in neurobiological research

• Formation of embryoid bodies in stem cell research

• Research on activation and deactivation mechanisms in lymphocytes, monocytes, macrophages, and other phagocytic cells.

• Suspension cell-based screening.

• Culture of cells, tissues, or organ fragments.

• Formation of multicellular spheres in tumor research.